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Restek Corporation
United States
Приєднався 2 лис 2007
Restek is a leading developer and manufacturer of chromatography columns and accessories. We provide analysts around the world with the innovative tools they need to monitor the quality of air, water, soil, foods, pharmaceuticals, chemical, and petroleum products.
Restek at analytica 2024
We just returned from another fantastic analytica. Waffles, a quiz, and plenty of chromatography. If you were able to join us, thank you! And if not, you can see a little of what you missed and make your plans for the next analytica!
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Connect with Us
UA-cam: ua-cam.com/users/RestekCorp
LinkedIn: www.linkedin.com/company/restek-corporation/
Facebook: RestekCorporation
Переглядів: 194
Відео
Syringe Care and Maintenance for Chromatographers
Переглядів 2,5 тис.Рік тому
Many chromatographers rely on syringes every day to do their work. They need to be accurate and consistent, but also reliable and dependable whether you use them manually or in an autosampler. In this Restek Tip, we cover syringe care and maintenance. What steps can you take to guarantee a long life for your syringe? And how should you properly maintain it to keep it in tip-top condition? Addit...
LC UV Detector Issues
Переглядів 1,8 тис.Рік тому
UV detectors are the most common type used in liquid chromatography. Many of us rely on them every day, but if you start to notice errors in your chromatogram, there could be a problem with your detector. In this Restek Tip, we look at some of the issues you might encounter with a UV detector. What should you look out for and what troubleshooting steps can you take? Additional Resources Effecti...
Syringe Basics for Chromatography
Переглядів 2,7 тис.Рік тому
From sample preparation to sample injection, syringes cover a range of uses in every chromatography lab. But with so many types and options available, how do you know which one to choose? In this Restek Tip, we guide you through a straightforward decision-making process to help you choose a new syringe. Contents 0:00 Choosing A New Syringe 0:19 Consider Your Application 0:44 Gas Tight or Liquid...
LC Leak Checking
Переглядів 2 тис.Рік тому
Leaks are one of the most common problems users encounter when performing liquid chromatography. Even the smallest leak in your LC system can cause a loss of sample, a drop in efficiency, and break your entire analysis workflow. In this Restek Tip, we investigate the signs of a possible leak and outline the troubleshooting steps you should take if a leak is discovered. Additional Resources Effe...
Pro EZLC Software Simplifies LC Method Development
Переглядів 1,4 тис.Рік тому
LC method development typically involves a lot of trial and error. It occupies precious lab resources and can take a considerable amount of time. Our Pro EZLC chromatogram modeler dramatically speeds up LC method development. Add analytes, test column chemistries, and optimize run conditions virtually and instantly-without needing to make a single injection. And it’s completely free to use. Add...
When and Why to Use an Electronic Flowmeter
Переглядів 2,6 тис.Рік тому
Flowmeters are often seen as essential tools for gas chromatography labs. But, since modern GC instruments feature advanced pressure controls, valves, and built-in flow measurement software, is it necessary to have a handheld electronic flowmeter device? In this Restek Tip, we share some situations where an electronic flowmeter can be used and look at the benefits of using one instead of your i...
What Do You Do with a New LC Column?
Переглядів 11 тис.Рік тому
Before you can use a new LC column to perform analyses, there are a few important steps you need to do to prepare it for its new life in your lab. In this Restek Tip, we show you how to set up your new LC column. We cover certificate of analyses (CofAs), discuss the different column end-fittings you can use, share how to condition your column for your mobile phase, and finally, we’ll look at ho...
Optimizing Split Injections
Переглядів 6 тис.2 роки тому
When performing a split injection, there are several considerations to keep in mind to improve the quality of your chromatography. But what should you change and why? In this Restek Tip, we’re going to show you how to optimize your split injections. We’ll start with liner selection, before discussing the importance of split ratios. Next, we’ll focus on optimizing the inlet temperature, and fina...
3 Ways to Reduce Your Helium Usage
Переглядів 3,4 тис.2 роки тому
Helium scarcity continues to be a pain point for gas chromatography. Prices are trending upward, supplies can be hard to source, and it’s a problem that isn’t going to go away. In this Restek Tip, we share three different ways to reduce your gas usage to get the most out of your helium cylinders. Chapters 0:00 The Trouble with Helium 0:23 Leak Checking Your GC 1:05 GC Gas Saver Mode 2:22 Shutti...
What You Need to Know About Gas Regulators
Переглядів 10 тис.2 роки тому
Gas regulators are fascinating devices and necessary tools in many chromatography applications. But with many options available and multiple variables to consider, choosing the right one can be a difficult task. In this Restek Tip, we share what you need to know about gas regulators. What are the differences between them? And what should you consider when selecting one? Additional Resources How...
Installing the SPME Arrow Conversion Kit
Переглядів 2,2 тис.2 роки тому
The SPME Arrow is a robust and versatile sample preparation device. To take full advantage of its many benefits, you first need to configure your GC. In this Restek Tip, we outline the parts you need, show you how to correctly install them, and answer some common questions. Solid phase microextraction, or SPME, is a fast and versatile sample preparation technique that reduces sample handling an...
What is SPME?
Переглядів 16 тис.2 роки тому
Solid phase microextraction, or SPME, is a fast and versatile sample preparation technique that reduces sample handling and solvent consumption. It can be used for headspace and immersion sampling while producing very clean extracts. In this Restek Tip, we explore the basics of SPME. What is it? How does it work? And what are its advantages? Additional Resources High-Performing Restek PAL SPME ...
Optimizing Splitless Injections
Переглядів 19 тис.2 роки тому
Splitless injections excel at trace analyses, but to get to get the most out of a splitless analysis, it’s important to optimize your system. But what should you optimize and what are the benefits? In this Restek Tip, we’re going to show you how to optimize your splitless injections. We’ll start with liner selection, before discussing the importance of the splitless hold time. Next, we’ll focus...
Why Are There so Many C18 LC Columns?
Переглядів 11 тис.3 роки тому
From end-capped to aqueous, there are a multitude of C18 phases to choose from. But why are there so many? A C18 is a C18, right? In today’s Restek Tip, we’re going to explore the world of C18 phases. Are there benefits to choosing one specific to your analysis? And what parameters should you keep in mind when selecting a phase? Additional Resources A C18 is a C18, right? | www.restek.com/chrom...
Rapid Screening & Confirmation of Analytes in Biofluids Using a Single Sample and Sampling Device
Переглядів 9953 роки тому
Rapid Screening & Confirmation of Analytes in Biofluids Using a Single Sample and Sampling Device
LC-MS/MS Analysis of Glyphosate & Other Polar Contaminants in Food with an Ion Exchange/HILIC Column
Переглядів 2 тис.3 роки тому
LC-MS/MS Analysis of Glyphosate & Other Polar Contaminants in Food with an Ion Exchange/HILIC Column
Automated In-Line Pigment Removal for Multi-Residue Pesticide Analysis in Spinach by LC-MS/MS
Переглядів 1 тис.3 роки тому
Automated In-Line Pigment Removal for Multi-Residue Pesticide Analysis in Spinach by LC-MS/MS
Analysis of the California List of Pesticides and Mycotoxins in Edibles
Переглядів 1,6 тис.3 роки тому
Analysis of the California List of Pesticides and Mycotoxins in Edibles
Coated Blade Spray-Tandem MS for Rapid Screening & Quantitation of Drugs in Oral Fluids
Переглядів 1 тис.3 роки тому
Coated Blade Spray-Tandem MS for Rapid Screening & Quantitation of Drugs in Oral Fluids
Preparing a New Reference Standard for Use
Переглядів 8 тис.4 роки тому
Preparing a New Reference Standard for Use
Correctly Using a Press-Tight Connector
Переглядів 4,2 тис.4 роки тому
Correctly Using a Press-Tight Connector
Connecting GC Columns Using a SilTite µ-Union
Переглядів 5 тис.4 роки тому
Connecting GC Columns Using a SilTite µ-Union
You guys are explaining this so good. I use your video's to show my students about how to troubleshoot!
Wow, thank you!
Thank you for such wonderful explanation.
Nice video! this is in my school materials, i could see what bleeding is on a graph but couldn't put it in the right words. Now i know that it is additional unwanted signals in the graph caused by the degradation of the stationary phase caused by a reactive chemical like a strong acid or base or by oxygen contamination so it is very important to filter for oxygen in the carrier gas.
Hi, thank you for the video! Can you tell me a little bit about the difference between a fixed flow regulator and a variable flow regulator?
Hello, how much is the value of the lamp based on its voltage?
Thanks that was very helpful. Please make a video about maintaining the ECD detector. thanks
After how many injections, (on average), should the filter be replaced? And what about cleaning the split vent lines?
This can vary greatly. As a default, we'd always recommend checking with your instrument manufacturer's guidelines. These are generally around every six months. How much you are injecting, how much is your split, how dirty are your samples can all impact this. Cleaning split vent lines is generally reserved for a situation where you feel it is needed, such as seeing some ghost peaks or other evidence of contamination. In an extreme case, when you notice incorrect flow rates and may suspect some clogging.
Obrigado
Sou de São Paulo Brasil e trabalho com um cromatógrafo com duas colunas e quando injeto a mesma amostra nos dois, tem muita diferença no resultado, e sinal do fid do front e 6,2 e do back está em 7,8. vou fazer um vídeo e mostrar aqui nos comentários ok.
Obrigado pela informação
Muito obrigado amigo, moro em São Paulo Brasil, e trabalho com um cromatógrafo com duas colunas e uma está com o sinal (fid) muito alto, o front está com 6,2 e o back com 7,8, quando injeto a mesmo amostra nos dois, dá uma diferença, e também no final da corrida e sinal aumenta muito. Vou fazer um vídeo mostrando o ocorrido.
Thank you so much!
The blank must be the same as the mobile phase?
Hi, can you show us how to replace the frit Agilent 1290 system?
do you offer the split vent trap for shimadzu GC2030? The new design for 2030 is offered at hilariously outrageous $200/unit.
Hello, I'm sorry, this is not a product that we offer. Thank you for asking!
You talk about the initial oven temperature, does this mean that the separation was carried out in temperature programming mode? Tank you.
Generally yes, but it doesn't need to be.
Can you help me, the back detector shows temp not installed. And my infront gas (N2) flow rate i set is 45, but the screen show range 51 - 60. Can you help?
*Make video on TCD*
I have faced an ignition cut-off when the sample was injected. Upto injection of sample flame is on and shows a good signal. but when injected immediately, the ignition will cut. what is the reason? can you please tell me?
What are you injecting and how much? You may wish to troubleshoot by trying to inject less or a different solvent. You can also do an no injection instrument blank to see if it is a timing/flow issue rather than the injection itself. You can find instructions on how to do that here ua-cam.com/video/tpebPhvQERY/v-deo.htmlsi=DNIgtobWEzHsxpe3
I have an Agilent ZORBAX NH2 and Carbohydrate column which is used for HILIC separation for sugar. Since those 2 columns have not been used for a period of time. Which solvents are suitable to use for cleaning and regeneration?
For any specific column, we would always recommend referencing the original manufacturer's instructions. Here is some information that may be helpful www.agilent.com/cs/library/datasheets/public/820629-008D.pdf For general information on LC column regeneration, we have a nice blog post www.restek.com/chromablography/resurrecting-an-old-reverse-phase-lc-column--should-i-could-i-how-would-i
Can you explain what's the purpose of the serrated edge, please? (Assuming there is a purpose).
In the past, the serrated edge was recommended for use for cutting some smaller ID metal columns. Generally, even those are now recommended to be cut with the smooth edge.
@@RestekCorp Thank you for the information.
What is the makeup gas?
Whis is mechanisms of FID detector
Could you please make a complete video for working Software and analysis details step by stem
How to remove the flowcell contamination?
What is difference between RI,PDA,Uv dectors
How to reduce the column back pressure?
Which solvent suitable for c18 columns?
How to remove the column blockage?
Can one extrapolate two neighbor peaks with resolution below 1.0? Is it correct to integrate the area of extrapolated peaks that are artificially deconvoluted?
Pl.tell about collector assembly sir
What is the principal behind FID Jet
How to clean the FID jet and how to know jet is damaged,?
Good Explanation sir.
What is the minimum syringe injection volume during method optimisation
We are use mostly Exmair syringe.After 100 injection , syringe response wasn't good.What is the best syringe and rugged
What basis syringe(10 micro or 5 micro litre) to be chosen based on injection volume 1 micro litre
Why syringe is struck after 8 injection of DMSO mostly
Thank you for ur guidance ...it's really helpful...thank you...sir
We got solvent peak but no sample peak. Any advice for this? Thanks! Appreciate your assistance.
I also encounter the same issue.
What about chemicals with labile hydrogen such as carboxylic acid due to the column ? Is there a way to avoid peak tailing ?
Compounds like carboxylic acids, can be very difficult to get good peak shapes. Depending on the column phase, there may be columns specifically designed to work with acids that can help. Stabilwax-DA columns are an example. Derivitization is also used for some compounds to improve peak shapes - fatty acids are often methylated before being analyzed for this reason. Some analysts also do priming injections - injecting high concentration standards to help occupy active sites before doing their analytical injections. There are many options, but they can all have their pros and cons.
Congratulations for the video, but I don't understand how a pulsed split injection can have the liner with increased pressure if the injector outlet opens?
The pressure controls of the GC system are designed to handle these pressure changes. So while there is still flow going through the column (and out the split vent, septum purge, etc.) pulsed injection allows users to temporarily elevate the pressure in the injection port.
@@RestekCorp Great! Thank you for this.
You're welcome!
sir why do you need to cut the column? what is the purpose of cutting a column?
Came to watch how to properly re-install the collector assembly. I also am having a hard time.
I am studying for my Chromatography course and this video was literally so well made. Thank you!
Hi sir, In case of benzene in carbomer as per USP method ... the benzene standard getting deterioration after 1 or 2 injections through splitless mode where i have been connected with split line in the GC...is this problem are having connection with split liner...
Could anyone recommend me packed column can separate oxygen, nitrogen and CO to tcd?
Where can I get the cap for the septum purge?
That will depend on your instrument. Many can use standard compression fitting caps, but you should check your instrument manual or instrument manufacturer to be sure.
I'm careful if I can ask here, but is it possible to analyze isophthalic acid by GC-MS? I have been having a tailing problem for a month. I will buy all the products you recommend. Somebody help me T^T
Tailing happens when the analyte of interest retains in the column. How the oven is programmed, gas flow rate, or the column used could impact your acid. You might need to look into different columns since you are dealing with aromatic acids, like Chromaton N-AW-HMDS with 5% Apiezon L column.
Each video is too short,Can some long videos provided to explain the details?